PTTG1IP Knockout cell line (U-251 MG)

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PTTG1IP Knockout cell line (U-251 MG) Order

PTTG1IP Knockout cell line (U-251 MG)
Catalog#
YKO-HT12752
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
PTTG1IP Knockout cell line (U-251 MG)
Order

Detailed Product Information

Product category
KO Cell Lines
Cell name
PTTG1IP Knockout cell line (U-251 MG)
Species
Human
Gene Name
PTTG1IP
Gene ID
754
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the PTTG1IP gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the U-251 MG cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, PTTG1IP gene knockout PTTG1IP Knockout cell line (U-251 MG) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
PTTG1IP Knockout cell line (U-251 MG)
PTTG1IP Knockout cell line (U-251 MG)
Catalog#
YKO-HT12752
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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  • PTTG1IP Knockout cell line (Jurkat)

    Organism: Human
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  • PTTG1IP Knockout cell line (Hep G2)

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